PAP-1

Catalog No.E0378 Batch:E037801

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Technical Data

Formula

C21H18O5

Molecular Weight 350.37 CAS No. 870653-45-5
Solubility (25°C)* In vitro DMSO 70 mg/mL (199.78 mM)
Ethanol 25 mg/mL (71.35 mM)
Water Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description PAP-1 (5-(4-phenoxybutoxy)psoralen), a Kv1.3 antagonist with an EC50 of 2 nM, inhibits the proliferation of human T cells and suppresses delayed type hypersensitivity in rats.
Targets
Kv1.3 [1]
2 nM(EC50)
In vitro

PAP-1 (2-100 nM; 30 minutes) suppresses the proliferation of CCR7-TEM cells with IC50 of 10 nM.[1]

In vivo

PAP-1 (0.3-3 mg/kg; i.p.; three times daily for 48 hours) prevents delayed type hypersensitivity (DTH) in lewis rats.[1]

Protocol (from reference)

Cell Assay:

[1]

  • Cell lines

    CCR7-TEM cells

  • Concentrations

    2-100 nM

  • Incubation Time

    30 min

  • Method

    CCR7- T cells are then seeded at 5 X 104 per well together with 5 X 104 autologous irradiated peripheral blood mononuclear cells in RPMI culture medium into round-bottomed, 96-well plates. PAP-1 is added at different concentrations, and the cells are stimulated with 25 ng/ml soluble anti-CD3 Ab 30 min later. Cells are harvested onto glassfiber filters, and radioactivity is measured in a ß-scintillation counter.

Selleck's PAP-1 has been cited by 2 publications

Immunosuppressive effects of new thiophene-based KV1.3 inhibitors [ Eur J Med Chem, 2023, 259:115561] PubMed: 37454520
Bergapten attenuates microglia-mediated neuroinflammation and ischemic brain injury by targeting Kv1.3 and Carbonyl reductase 1 [ Eur J Pharmacol, 2022, 933:175242] PubMed: 36058290

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.